■ 基本信息
別名: | pBAD-18 |
啟動(dòng)子: | araBAD |
復(fù)制子: | pBR322 |
質(zhì)粒分類: | 大腸桿菌載體；pBAD系列表達(dá)質(zhì)粒 |
質(zhì)粒大小: | 4613bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，有氧，LB |
表達(dá)宿主: | 推薦LMG194 |
誘導(dǎo)方式: | 阿拉伯糖 |
5'測(cè)序引物: | pBAD-F: ATGCCATAGCATTTTTATCC |
3'測(cè)序引物: | pBAD-R: GATTTAATCTGTATCAGG |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 大腸桿菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF |
片段物種: | 空載體 |
原核抗性: | Amp |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
This vector is one of several tightly controlled expression vectors regulated by the arabinose operon. Vectors differ in replicon, antibiotic resistance marker, multiple cloning site and need for translation initiation sequences. Cultures should be grown in minimal media for more reproducible induction of expression. Expression is induced in glycerolcontaining media by the addition of arabinose. Expression is repressed by addition of glucose or other catabolites. Cloned inserts must provide a translation initiation sequence (ATG) and ribosome binding site for expression. Expression of cloned inserts in the two recommended hosts differ in terms of induction characteristics, especially when using low concentrations of inducer (arabinose) and growing in minimal media. The following primers can be used for sequencing of cloned inserts: 5 primer (278bp upstream of the NheI site) :5 CTGTTTCTCCATACCCGTT 3and one of two 3 primers:3 primer 1 (219bp downstream of the HindIII site):5 CTCATCCGCCAAAACAG 33 primer 2 (1733bp downstream of the HindIII site):5 GGCTGAAAATCTTCTCT3
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK1604 pBAD18大腸表達(dá)質(zhì)粒.txt
