■ 基本信息
別名: | pGL3.0-Enhancer |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 哺乳細胞，信號通路報告載體 |
質(zhì)粒大小: | 5064bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物: | RVP3:CTAGCAAAATAGGCTGTCCC |
3'測序引物:
| 根據(jù)序列設計引物
|
備注: | 哺乳細胞啟動子報告對照質(zhì)粒 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 信號報告 |
片段類型: | Promoter
|
片段物種: |
|
原核抗性: | Amp |
真核抗性: |
|
熒光標記: | Fluc
|
■ 質(zhì)粒簡介
The pGL3 Luciferase Reporter Vectors(a–c) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL3 Luciferase Reporter Vectors is designed for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, these Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK470pGL3-Enhancer哺乳報告質(zhì)粒.txt
