■ 基本信息
別名: | pGL3.0-Basic |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質粒分類: | 哺乳細胞，信號通路報告載體 |
質粒大小: | 4818bp |
原核抗性: | Amp |
真核抗性: | 熒光素酶Luc |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物:
| RVP3:CTAGCAAAATAGGCTGTCCC
|
3'測序引物: | GLP2:CTTTATGTTTTTGGCGTCTTCC |
■ 質粒屬性
質粒宿主: | 哺乳細胞 |
質粒用途: | 信號報告 |
片段類型: | Promoter
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
|
熒光標記: | Fluc
|
■ 質粒簡介
The pGL3 Luciferase Reporter Vectors(a–c) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL3 Luciferase Reporter Vectors is designed for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, these Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK467pGL3-Basic哺乳報告質粒.txt
