■ 基本信息
別名: | Plasmid #63892 |
啟動(dòng)子: | Lac/lac promoter |
復(fù)制子: | pUC |
質(zhì)粒大小: | 4772bp |
質(zhì)粒標(biāo)簽: | C-6×His，C-HA |
原核抗性: | Amp |
克隆菌株: | TG1 |
培養(yǎng)條件: | 37度 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 大腸桿菌,噬菌體 |
質(zhì)粒用途: | 蛋白表達(dá),抗體表達(dá),噬菌體展示 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: |
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■ 質(zhì)粒簡介
Backbone pComb3X containing PCR template for human antibody lambda light chain constant region.
pComb3X is the newest of the pComb vectors. Improvements over pComb3 include increased stability and introduction of an asymmetric SfiI cassette for directional cloning of full Fab, scFv, peptide and other protein for phage display. 6xHis and HA tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest. pComb3XLambda can be used as a template to amplify the lambda light chain constant region for construction of chimeric antibody libraries as described in:
Barbas, C. F., III; Burton, D. R.; Scott, J.K., Silverman, G.J. Eds. (2001) Phage Display: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York
Please note that there is a A246V mutation in heavy chain region; however, this vector is meant to be used as a PCR template for the light chain region from base# 613-1016.
本質(zhì)粒參考文獻(xiàn)：Methods for the generation of chicken monoclonal antibody fragments by phage display.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列可參考（具體以測序?yàn)闇?zhǔn)）下載：
ZK128pComb3XLambda噬菌體展示質(zhì)粒.txt
